Iron overload favours the elimination of Leishmania infantum from mouse tissues by enhancing the production of reactive oxygen species
Abstract number: P963
Costa S., Pereira S., Teixeira C., Tomás A., Appelberg R., Gomes S.
Objectives: We have previously determined that iron overload decreases the growth of Leishmania infantum in the liver and spleen of a susceptible mouse strain. The purpose of this work was to investigate if the inhibitory effect of iron overload on the growth of this protozoan is dependent on the production of reactive oxygen species by the macrophagic NADPH oxidase.
Methods: To achieve this goal, we used mice genetically deficient in the p47phox subunit of NADPH oxidase (p47phox knock-out). Control and knock-out mice were treated with iron-dextran (10 mg) or saline solution and 10 days later were infected with L. infantum. Mice were sacrificed two weeks after infection and the parasite load was determined in the liver and spleen.
Results: In both organs, iron overload decreased L. infantum growth in control mice but not in knock-out mice, indicating that the mechanism through which iron exerts its inhibitory effect on the growth of the parasite is dependent on the production of reactive oxygen species by the host cell.
Conclusion: Iron overload inhibits L. infantum growth in mice through the production of reactive oxygen species by NADPH oxidase, a potent antimicrobial mechanism to which Leishmania is susceptible.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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