Molecular typing indicates an important role of two international clonal complexes in the dissemination of VIM-producing Pseudomonas aeruginosa clinical isolates in Hungary
Abstract number: P902
Libisch B., Balogh B., Paszti J., Szabo G., Gacs M., Fuzi M.
Objectives: VIM metallo-b-lactamase-producing serotype O11 or O12 Pseudomonas aeruginosa isolates infecting or colonising 19 patients from seven hospitals were reported from Hungary between 2003 and 2005. In this work we identified VIM-producing Pseudomonas spp. strains from Hungarian towns and hospitals where these resistance determinants had not been detected before. Our aim was to characterise these isolates by molecular and phenotypic tools and to investigate their epidemiological relationship.
Methods: We screened the isolates by EDTA-inhibition based phenotypic tests. blaVIM genes and class 1 integrons were detected by PCR and were sequenced. PFGE was performed by the method described by Poh et al. with modifications and MLST was carried out according to the protocol published by Curran et al.
Results: The newly identified P. aeruginosa MBL producing isolates could be assigned to serotypes O1, O11 and O12. All isolates proved multidrug-resistant; however, it is notable that all P. aeruginosa isolates remained susceptible to aztreonam by the current CLSI breakpoints. VIM-producing Pseudomonas spp. clinical isolates from two novel locations and hospitals in Hungary were identified, with the detection of three new blaVIM carrying integron types and the presence of the blaVIM-2 allele in Hungary. By applying various typing techniques including multilocus sequence typing we revealed an important role of two international clonal complexes in the dissemination of blaVIM positive P. aeruginosa in hospitals in Hungary. We characterised a P. aeruginosa nosocomial clone with a singleton sequence type (ST313), too, that could have acquired blaVIM-2 and blaVIM-4 gene cassettes from a yet unidentified local gene pool in Hungary.
Conclusions: This is the first report of the occurrence of P. aeruginosa isolates from Hungary belonging to an international clonal complex initially described as BG11. In this study we have demonstrated a high diversity of MBL carrying integrons in Hungary and also propose that a combination of different factors, such as clonal spread and local acquisition of MBL genes may both contribute to the appearance of VIM positive Pseudomonas spp. strains in our country. This work was supported by the DRESP2 FP6 project.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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