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Importance of molecular detection for diagnosis and monitoring mumps-virus infection

Abstract number: P775

Royuela E., Castellanos A., Guisasola M.E., Sanz J.C., Mosquera M., Sanchez-Herrero C., De Ory Manchón F., Echevarría J.E.

Objectives: Mumps-virus (MVs) is a paramyxovirus that causes fever, headache and swelling of the salivary glands. Complications such as meningitis, encephalitis and orchitis are relatively frequent, however permanent sequelae are rare. Routine vaccination included in immunisation schedule has resulted in a dramatic drop in the number of reported mumps cases, although, outbreaks in vaccinated population have recently occurred, including large outbreaks worldwide. World Health Organization (WHO) recommendations for diagnosis of mumps infection includes isolation of MVs from clinical specimens or seroconversion (or significant rise in serum mumps IgG titre) or positive serological test for mumps-specific IgM antibodies. These recommendations do not include molecular detection using RT-PCR. Our goal was to evaluate the importance of RT-PCR detection methods for MVs detection in clinical samples.

Methods: For that reason, we developed an study including serological and RT-PCR detection methods, in which 593 complete cases were analysed (complete cases include serum and saliva and/or urine samples).

Results: From these analysed samples, 296 (49.9%) were positive, being 30.7% IgM and RT-PCR positive, 29.7% IgM positive but RT-PCR negative and a surprising 39.5% IgM negative but RT-PCR positive. This 39.5% positive to RT-PCR showed 97.43% IgG positive, indicating a secondary vaccine failure since reinfection was discarded after analysis of patients vaccination antecedents.

Conclusions: Data presented in this study demonstrate the importance of molecular detection methods for diagnosis of MVs infection and for the evaluation of vaccine failure previously reported, since using only serological methods the MVs infection incidence is understimated.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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