Influence of combined contraceptive vaginal ring on vaginal microflora and immunity

Abstract number: P760

Orlova V., Naberezhnev Y., Lebedeva O.

Objectives: to establish influence of vaginal ring on microbiocenosis of low female reproductive tract.

Methods: 31 female women age of 20–25 years old were examined before and after 3-month usage of contraceptive vaginal ring (NuvaRing). Vaginal ring is combined low-dosed hormonal contraceptive which releases 120 mg of etonogestrel and 20 mg of ethinyloestradiol daily. The samples of vaginal discharge were inoculated into a culture medium (Columbia base agar, blood agar, Man Rogosa Sharpe medium, semisynthetic growth medium, McConkey agar, Gardnerella vaginalis agar and Sabouraud agar) and cultured for anaerobic and aerobic bacteria. Secretory immunoglobulin, IFN-gamma, IL-4, lysozyme in cervico-vaginal mucus were detected by ELISA. Data were analysed using Statistica 6.0 (StatSoft, USA).

Results: After vaginal ring usage considerable changes of vaginal microbiocenosis structure were observed. Amount of Lactobacillus increased from 104 to 106 colony forming units (CFU) and Bifidobacterium from 18.2±1.2 to 44.4±6.1 CFU (p leqslant R: less-than-or-eq, slant 0.05). Percentage of women, having three-component microbial association (Peptostreptococcus, Staphylococcus and Streptococcus) decreased from 66.7±8.8% to 46.7±9.1%. Nevertheless, number of Candida albicans increased from 102 to 104 which gave rise to symptomatic yeast infection.

Elimination of cocci during NuvaRing usage could be consequence of elevation of secretory immunoglobulin (from 47±12.5ng/ml to 94.5±22.1 ng/ml) and IL-beta (from 95.7±29.1 pg/ml to 130.2±29.1pg/ml) (p leqslant R: less-than-or-eq, slant 0.05). High rate of Candida detection, apparently, could be result of high local level of estrogen among women using contraceptive vaginal ring.

Conclusion: Thus, our studies showed increasing concentration of lactobacilli and bifidobacterii, and cocci elimination, wich is favourable for antiinfectious resistance of female reproductive tract. Meanwhile, high risk of yeast infection appearance doesn't make possible to recommend this type of contraception for women with recurrent candidiasis, diabetus mellitus, AIDS and etc.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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