Detection of amebiasis among men who have sex with men, with or without human immunodeficiency virus infection

Abstract number: P742

Hung C.C., Ko N.Y., Wu P.Y., Wu C.H., Liu W.C., Sun H.Y., Chang S.C.

Objectives: Men who have sex with men (MSM), regardless of HIV status, are at increased risk for amebiasis because of oral-anal sexual contact in Taiwan. In this study, we aimed to evaluate the association of seroconversion for Entamoeba histolytica infection with newly acquired intestinal infection with E. histolytica among MSM.

Methods: Between 1 January, 2007 and 30 November, 2007, 143 MSM (128 HIV-infected) who were free of gastrointestinal symptoms provided both blood and stool specimens for serological tests and specific amebic antigen tests for E. histolytica infection, respectively. Anti-E. histolytica antibodies were determined using indirect hemagglutination (IHA) assay (Cellognostics, Boehhringer Diagnostics GmbH, Marburg, Germany), while stool amebic antigen was determined using commercial test kits (ENTAMOEBA TEST, TechLab, Branchburg, NJ) followed by polymerase chain reactions (PCR) using specific primers for E. histolytica.

Results: In this survey, the prevalence of intestinal E. histolytca infection was 9.1% (13/143) and the seroprevalence of E. histolytica infection (IHA titers leqslant R: less-than-or-eq, slant32) was 17.5% (25/143) among the subjects. MSM who were seropositive were at statistically significantly higher risk for intestinal E. histolytica infection with an odds ratio of 25.56 (95% confidence interval, 6.316, 103.4) (p < 0.0001).

Conclusion: We found that MSM who were IHA-seropositive for E. histolytcia was more likely to present with intestinal E. histolytica infection than those who were seronegative. In clinical settings where amebiasis is suspected and stool samples may not be readily available, serologies for E. histolytica is a cost-effective screening alternative to identify E. histolytica infection among MSM.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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