Genotyping of Trichosporon asahii strains isolated from urinary tract infections in a Turkish university hospital

Abstract number: P733

Karahan Z.C., Koyuncu E., Dolapci I., Arikan Akan O., Tekeli A.

Objectives:Trichosporon infections are associated with a wide spectrum of clinical manifestations ranging from superficial cutaneous involvement to severe systemic disease depending on the immune status of the patient. Trichosporon asahii appears to be the most important species in the genus. Invasive infections with T. asahii has been described most frequently in immunocompromised hosts, although some case reports of T. asahii infections in immuncompetent patients exist. In this study, we evaluated the genotypic relatedness of T. asahii strains isolated from non-immunocompromised patients' urinary tract infections.

Methods: A total of 25 T. asahii urinary strains were studied. The isolates were obtained from17 immuncompetent patients (11 male, 6 female, median age 44) hospitalised at different wards (neurosurgery, urology, nephrology, orthopaedics, reanimation) of Ankara University Ibn-i Sina Hospital between July 2004-April 2005. None of the patients were neutropenic during the period when T. asahii was isolated. T. asahii identification was made by using the API 20C AUX (BioMérieux Vitek, USA) and confirmed by PCR by using genus and species specific primers. Genotyping of T. asahii strains were made by RAPD and REP-PCR methods by using M13 and ERIC2 primers, respectively.

Results: All of the isolates gave amplicons by Trichosporon specific primers. When species specific PCR was performed, 23 of the isolates obtained from 15 patients gave amplicons. These 23 isolates were subjected to genotyping. RAPD analysis by M13 primer resulted in clustering of the isolates into 5 groups; four of the groups were composed of single isolate, and the fifth group contained 19 isolates. The results of REP-PCR analysis showed good corelations with the data obtained by RAPD method, although this method produced three different groups. Two of the groups contained single isolates which corresponded to the two groups of RAPD analysis. The third group consisted of 21 isolates containing the two isolates which were distributed to different groups by RAPD analysis.

Conclusion: Here we report 15 immuncompetent patients with urinary tract infections due to Trichosporon asahii. In this first study of invasive trichosporonosis from Turkey, 19 of the 23 isolates were found to be genotypically related, suggesting that a single genotype was prevalent in the hospital environment. The genotypically unrelated four isolates were probably of endogenous origin.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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