Genotypic relatedness of Candida spp. in neonatal intensive care unit
Abstract number: P727
Eimra K., Truusalu K., Järv H., Ilmoja M., Padari H., Lutsar I.
Objectives: We aimed to characterise the genetic relatedness of Candida species isolated from samples taken on clinical grounds in two NICUs in Estonia using pulsed field gel electroforesis (PFGE).
Methods:Candida spp. were isolated from August 2005 to May 2006 in unit A and from June 1999 to November 2005 in unit B and stored in sterile water or cryopreserved. The species were identified with CHROMagar Candida, Corn Meal Agar with Tween 80 and API Candida. The relatedness of the isolates was analysed by optimisation of previously described PFGE method as follows: BssHII digested DNA was electrophoresed with a voltage of 4V/cm, temperature 14 degrees C, linear ramped pulse times 5 to 50 seconds and run time of 20 hours.
Results: A total of 83 isolates (12 from blood, 57 from trachea and 14 from other sites) from 52 neonates were analysed. Of 16 invasive isolates C. parapsilosis (n = 8) and C. albicans (n = 6) were most commonly seen. C. guilliermondii (30%), C. parapsilosis (30%) and C. albicans (29%) were predominant isolates in colonised patients. In unit A all 6 C. albicans isolates were genotypically different whereas in unit B only 2 clones among 18 isolates were identified. Of 25 isolates of C. guilliermondii and C. parapsilosis each only 2 and 3 different clones, respectively, were seen. However, Candida strains isolated in 200001 differed from those obtained in 200506 by one or two bands suggesting that microevlolutionary changes within a strain had occurred.
Conclusion: Although C. guillliermondii was a frequent coloniser in the NICU patients, yet it was rarely associated with the invasive disease. The presence of a limited number of different clones of C. guilliermondii and C. parapsilosis indicates permanent source in the NICU and suggests for transmission by direct or indirect contact and cross-colonisation by healthcare workers.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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