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Prevalence of meticillin-sensitive and resistant S. aureus nasal colonisation in healthcare workers in a tertiary hospital

Abstract number: P656

Spyropoulos V., Moraitou H., Makarona M., Havouti F., Tsagarakis N., Kalikantzaros D., Lolis N., Kanavaki S.

Objectives: The aim of this study was to establish the rates of nasal carriage in medical and nursing personnel of a tertiary care Hospital for both meticillin-sensitive (MSSA) and resistant (MRSA) S. aureus, in order to perform infection control measures, if necessary. The MRSA strains isolated were further tested by PCR for the presence of mecA and pvl genes.

Methods: A total of 220 nasal swabs were taken from 111 healthcare workers participating in the study, of which 43 were medical (21 ICU clinicians and 22 medical microbiology doctors) and 68 non-medical staff (34 nursing and 34 laboratory personnel). All swabs were cultured onto Columbia Blood agar (Oxoid, UK) and ChromID MRSA agar (Biomerieux, France). All unique-patient S. aureus isolates were identified by VITEK 2 automated system and were further tested for antibiotic susceptibility by both VITEK 2 system as well as Kirby-Bauer disk diffusion method. All S. aureus isolates were further tested for the presence of mecA and pvl genes by PCR (Genotype Staphylococcus, Hain Germany).

Results: In total, the prevalence of S. aureus nasal carriage was 18.0%. MSSA carriage was 12.6% and MRSA 5.4%.

MSSA prevalence was 11.6% (5/43) among medical personnel and, in particular, 9.1% (2/22) for laboratory doctors and 13.6% (3/21) for ICU clinicians. MSSA prevalence among non-medical staff, was 13.2% (9/68) and in particular, 17.6% (6/34) for laboratory personnel and 8.8% (3/34) for ICU nursing personnel.

MRSA prevalence was 4.7% (2/43) among medical personnel, and in particular, 4.5% (1/22) for laboratory doctors and 4.7% (1/21) for ICU clinicians. MRSA prevalence among non-medical staff was 5.9% (4/68), 0% for laboratory personnel and 11.8% (11/34) for ICU nursing personnel.

All MRSA strains were found positive for mecA gene, by PCR, while only one strain was found to be pvl positive (5%, 1/20).

Conclusion: The prevalence of MSSA as well as MRSA nasal carriage is within the expected rates, as commonly mentioned in the literature. The highest MSSA rates were found among non-medical laboratory staff, while, the highest MRSA rates were found among ICU nursing personnel. Among medical personnel, the prevalence rates were similar, for both MSSA and MRSA. This study is a part of an ongoing epidemiological surveillance conduced in our Hospital, in order to eliminate MRSA carriage.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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