Serotype distributions and molecular characteristics of Vibrio parahaemolyticus isolates in Malaysia
Abstract number: P640
Shamsuddin F., Nadarajah V., Ahmad N., Md Yasin R., Nishibuchi M., Ishibashi M., Ong K.H.
Objectives: To determine the serotype distributions of Vibrio parahaemolyticus isolates in Malaysian clinical isolates and to identify the genotypic characteristics of the strains.
Methods: A collection of fourteen V. parahaemolyticus clinical isolates from various hospitals and National Public Health Laboratory in Malaysia from year 1997 to 2000 and 2005 to 2007 were serotyped using commercial O and K antisera. The isolates were subjected to PCR analysis to detect species-specific toxR gene of V. parahaemolyticus, tdh and trh toxin genes, presence of orf8 gene and confirmation of pandemic strain by group-specific PCR (GS-PCR).
Results: From the fourteen clinical isolates, eight were serotyped as O3:K6 making it the dominant serotype (57.14%), followed by O4:K68 representing three isolates (21.43%) and one strain each representing serotype O1:KUT, O3:K5 and O4:K8 respectively. All of these isolates were positive for the toxR gene and were confirmed as V. parahaemolyticus. All the isolates were also positive for the tdh toxin gene except for one strain of serotype O1:KUT. None of the isolates were positive for the trh gene. Only those of serotype O3:K6 and O4:K68 possessed all genotypic characteristics of the pandemic strain, being positive for the tdh gene and negative for trh gene, positive for the orf8 structural gene and were also GS-PCR positive. However, the three other serotypes were negative for orf8 gene and were GS-PCR negative.
Conclusion: This study showed that pandemic serotypes are present in Malaysia and are represented by serotype O3:K6 and O4:K68. In this limited number of isolates, O3:K6 and O4:K68 were shown to be the dominant serotypes. These particular strains possessed all structural genes of the pandemic strain which were further confirmed by GS-PCR method.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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