Molecular types of extended-spectrum b-lactamases in Escherichia coli strains isolated from community-acquired urinary tract infections in Turkey

Abstract number: P626

Arslan H., Kurt Azap O., Serin Senger S., Timurkaynak F.

Background: Extended-spectrum b-lactamase (ESBL)-producing Escherichia coli have been increasingly recognised in the community. The aim of this study was to determine the prevalence of CTX-M, SHV, and TEM types of ESBLs for community-onset ESBL-producing E. coli in urinary tract infections (UTIs).

Methods: ESBL-producing E. coli strains isolated from adults with community-onset UTIs during two distinct periods in 2004 and 2006 were collected from four centres in different cities of Turkey. The presence of ESBL was established on the basis of CLSI criteria and confirmatory tests. Polymerase chain reaction and DNA sequencing were used to characterise the blaTEM, blaCTX-M and blaSHV genes.

Results: We analysed 54 E. coli isolates (18 isolates from 2004 and 36 from 2006–2007). The prevalence of CTX-M, SHV, and TEM were 94.4% (51), 9.3% (5), and 42.6% (23) respectively. Sequence analysis of the blaCTX-M containing isolates revealed that these isolates contained two different blaCTX-M, 2 (3.9%) with blaCTX-M-3, and 49 (96.1%) with blaCTX-M-15. We found 3 different ESBL blaSHV types: blaSHV-12, blaSHV-5, and blaSHV-2. There were 2 blaTEM-116 carrying isolates.

The two isolates carrying TEM-116 had also CTX-M-15, and they belonged to the 2006–2007 period. There were no other significant differences in the two periods regarding the ESBL types. In the table the distribution of the CTX-M, SHV, and TEM ESBL types is shown.

Table: The distribution of ESBL types among the isolates

Conclusions: ESBL-producing E. coli in non-hospitalised patients seem to be emerging with various types and prevalence in different countries. Our study revealed dissemination of CTX-M type ESBL in E. coli strains isolated from community-acquired UTIs in Turkey. CTX-M-15 was the most common enzyme.

In this study in only two of the isolates we found ESBL-type TEM, and they were isolated in 2006. This may indicate that a novel ESBL type (TEM-116) has emerged. A close follow up whether this novel ESBL type disseminates would be appropriate. The coexistence of CTX-M-15 with ESBL-type TEM genes has been observed in two isolates, compatible with similar findings in recent studies.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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