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Identification of Mycobacterium tuberculosis-specific CD4 and CD8 IFN-gamma and/or IL-2 secreting cells by a novel method contributes to defining the different stages of tuberculosis

Abstract number: O305

Sargentini V., Mariotti S., Carrara S., Gagliardi M.C., Teloni R., Goletti D., Nisini R.

Objectives: A more detailed characterisation of the immune response to Mycobacterium tuberculosis (Mtb) antigens would provide new insights into the complex host-parasite interactions that take place in the course of tuberculosis (TB) and offer new tools for a more accurate diagnosis of the different stages that characterise TB as a disease.

Methods: Here we measured CD4 and CD8 T-cell responses to Bacillus Calmette-Guérin (BCG), purified protein derivative (PPD), early secretory antigenic target-6 (ESAT-6) protein and culture filtrate protein-10 kDa (CFP-10), measured as interferon (IFN)-gamma and interleukin (IL)-2 release, using the flow cytometric cells-secreting cytokine detection technique on peripheral blood mononuclear cells (PBMC) obtained from active TB patients, latently Mtb infected individuals, patients with pulmonary diseases different from TB and healthy donors. IL-10 and IL-17 were also measured to test their possible role as indicators of disease activity.

Results: We confirmed that the enumeration of IFN-gamma releasing cells upon Mtb-specific stimulation is sufficient to identify TB patients and that CD8 T cells contribute to the specificity of the response. IL-2 secreting cells were more frequently observed in latent TB infected individuals than in active TB patients, suggesting that measurement of cells secreting this cytokine could be a marker of disease stages. No discriminating role was associated to IL-10 and IL-17 release in TB patients.

Conclusions: Together, data indicate that the flow cytometric cytokine-secreting cell detection technique is a powerful tool for TB diagnosis which allows the analysis of the immune response to Mtb-related antigens in the different stages of TB.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Barcelona, Spain
Presentation type:
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