Human Bocavirus quantitative DNA detection and phylogenetic analysis in children hospitalised for acute bronchiolitis
Abstract number: O212
Andreoletti L., Jacques J., Renois F., Moret H., Motte J., Leveque N.
Objective: Human Bocavirus (HBoV) is a newly discovered parvovirus, but its role as causative agent of respiratory disease remains unclear.
Methods: We investigated the presence of HBoV by quantitative PCR of nasopharyngeal samples of 192 French consecutive children hospitalised for acute bronchiolitis. The detection of the other common respiratory viruses was performed using classical immunofluorescence antigens, cell culture detection, or RT-PCR assays.
Results: HBoV was the unique viral pathogen detected in 14 (7%) and was associated with another viral respiratory pathogen in 10 (5%) of 192 study children. This virus was identified as the third aetiological cause of bronchiolitis after respiratory syncytial virus and rhinovirus (45 (23%) and 24 (12%) of 192 cases, respectively), occurring more often in infants aged 112 months (P = 0.002). The median levels of the HBoV DNA genomes in respiratory samples appeared to be significantly higher in patients with single HBoV infection than those observed in patients with a mixed respiratory viral infection with HBoV (4.108 copies/ml vs. 2.103 copies/ml, P < 0.001).
Conclusion: Our data suggest that HBoV at a high viral load could be an aetiologic agent of respiratory tract disease, whereas the exact role of HBoV at a low viral load, as aetiological cause or as pathophysiological co-factor of respiratory diseases, remains to be determined.
|Session name:||18th European Congress of Clinical Microbiology and Infectious Diseases|
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