Prevalence and antibiotic susceptibilities of Yersinia enterocolitica and other Yersinia species recovered from meat and chicken in Tehran, Iran

Abstract number: O103

Golkar F., Soltan Dallal M.M., Baghai K., Azimi M., Zolfaghari K., Moezardalan S., Zali M.

Objectives:Yersinia enterocolitica is known as a psychrotrophic waterborne and food borne enteropathogen.Yersinia can grow to large numbers at refrigeration temperatures, so meat, chicken, milk, cheese contaminated with that organism could become a significant health risk for consumers. The aims of this study addition to investigate the prevalence of Y. enterocolitica and other Yersinia species in meat and chicken samples in different seasons, were to determine the antimicrobial susceptibility pattern of Yersinia enterocolitica and other Yersinia species isolated from meat and chicken in Tehran, Iran.

Methods: 189 peaces of meat and 190 chickens purchased from 28 different local butcher's shops and supermarkets in Tehran that examined for the presence of Yersinia species between April 2005 and March 2006.25 gr sample of homogenised food was pre-enriched in PBS medium then it was transferred to cefsulodin-irgasan-novobiocin (CIN) agar. Susceptibility testing of bacterial strains was achieved at 28°C by the agar diffusion method.

Results:Yersinia spp. was recovered from 60 of 379 (15.8%) peaces of meat and chickens samples. Y. enterocolitica was found in 48 of 60 (80%) positive samples. The other 3 Yersinia spp. were Y. frederiksenii, intermedia, kristensenii in 7 (11%), 4 (6%) and 1 (0.01%) of 60 isolates, respectively. Also the most prevalence of Yersinia spp. was in early spring and in mid autumn.

All 4 strains (98%) were susceptible to choloramphenicol and gentamicin and 95%, 86%, 78%, 76%, 63%, 41% were susceptible to trimethoprim and ciprofloxacin, cephotaxim, Tetracycline, nalidixic acid, streptomycin and ampicilin respectively. the most antibiotic resistance belong to cephalothine (98%).

Conclusion: Several factors such as isolation method, season, and geographical location play an important role in reports of increase or decrease in the prevalence of the Yersinia spp. Y. enterocolitica had the most prevalence among other species. Our results show isolation ratio of Y. enterocolitica and other species is higher in colder climates. The majority of isolates were resistant to first generation cephalosporins (cephalothine). The most active pharmacologic agents were choloramphenicol, aminoglycozide and sulfunamdes. Regarding to high Sensivity of Yersinia spp. to gentamicin and chloramphenicol, these agents should be effective in the treatment of Yersinia spp. when clinically indicated.

Session Details

Date: 19/04/2008
Time: 00:00-00:00
Session name: 18th European Congress of Clinical Microbiology and Infectious Diseases
Location: Barcelona, Spain
Presentation type:
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