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Phylogenetic, clinical and virological features of hepatitis B virus among Iranian HBsAg carriers

Abstract number: 1734_201

Bahramali G., Sadeghi-Zadeh M., Amini-Bavil-Olyaee S., Alavian S., Behzad-Behbahani A., Rabbani B., Sabouri E., Barkhordari F., Bagheri R., Mahboudi F.

Objective: Hepatitis B virus (HBV) infection is one of the major epidemiological problems around the world. Our previous reports indicated that genotype D of HBV was dominant one in Iranian HBV carriers. Herein, we have investigated a large number of cases in different stages of the disease to show phylogenetic, virological and clinical features of HBV in Iranian infected patients.

Methods: One hundred and twenty HBsAg carriers were selected from patients attending the hepatitis clinic centres. Liver function, clinical and serological status, viral load and HBV genome variability were analysed for each patient. The patients were categorised into four groups;18.8% inactive carrier patients, 45.4% chronic hepatitis B infected cases, 25.4% patients with cirrhosis and 10.9% with hepatocellular carcinoma (HCC).HBV genotype (HBsAg), BCP/Core and YMDD motif sequences were successfully amplified for Sixty two samples and sequenced in both direction.

Results: Phylogenetic analysis revealed genotype D and sub-genotype D1 with a high bootstrap value. Subtypes ayw2 and ayw3 were detected in 98% and 2% of samples, respectively. All patients with HCC and 64.5% of total patients were shown to be HBeAg-negative. The highest ALT rate was detected in patients with cirrhosis and HCC. There was no significant correlation between HBeAg negativity and ALT value in the studied cases. The prevalence of the precore mutation was 78% (70% in chronic infection patients, 20% in HCC patients and 10% in cirrhosis patients). The BCP double mutation was detected in 48% of the patients as well. Twenty-five percent of the patients who had lamivudine therapy showed mutation in the YMDD motif (50% among HCC patients, 33% in hepatitis B chronic carriers and 16.6% in cirrhotic subjects). No HBsAg escape mutant was detected in studied cases.

Conclusion: This work confirmed the earlier studies that the genotype D of HBV was the predominant one in Iranian patients. The rate of precore and BCP mutation was high among the studied cases and closely associated with HCC. The FLLA mutation was also found in patients who suffered from HCC. Nevertheless, no association was observed between ALT and HBeAg status. Analysis of these mutants may prove useful for clinical evaluation and choice of therapy.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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