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Hepatitis C virus genotypes distribution in infected patients

Abstract number: 1734_198

Suarez A., Alvarez M., Sanchez C., Picazo J.

Objectives: Hepatitis C Virus (HCV) is a frequent cause of chronic liver disease, and clinical features of HCV infection as well as responsiveness to treatment appear to vary with different genotypes, so the aim of this study was to evaluate the genotype distribution and identify its frequency when a Human Immunodeficiency Virus HIV infection is associated.

Methods: A total of 2176 patients infected by HCV were tested for genotype, 503 of them were co-infected with HIV.

HCV genotyping was carried out in all patients using a reverse transcription-nested polymerase chain reaction (PCR) technique, and a reverse hybridisation system of the amplified product (Inno-Lipa. Innogenetics) The protocol was performed and interpreted according with the recommendations of the manufacturer.

Clinical diagnoses were confirmed by histopatological analysis.

Results: The patients had a mean age of 53.4±10.6 years (range 25–82) and 65% were males, (co-infected patients: mean age of 36.3±6.1 years, and 84% males). HCV type 1 was detected in 1612 patients, in particular 1127 cases showed subtype 1b, type 2 in 42 cases, type 3 in 311 cases, type 4 in 176, type 5 in 8 cases, only one patient with type 6 and eight patients were infected with more than one genotype. Among the 503 HIV-co-infected patients (23.1%), the distribution of HCV genotypes was as follows: type 1 in 292 (19.3% of genotype 1b), 25.3% genotype 3, 14.9% genotype 4, 0.2% genotype 2 and none genotype 5 or 6. Five patients had mixed genotypes.

All patients suffering from hepatocellular carcinoma were infected by genotype 1b and 73% of the alcoholic cirrhosis patients. Genotype 1b was higher in non-co-infected patients (61.6% versus 19.3%), while genotypes 1a, 3 and 4 were more frequently in HIV-co-infected patients (all differences were statistically significant p < 0.001). However there were not significant differences between genders or among risk factors in the distribution of HCV genotypes among the HIV co-infected patients.

Conclusion: A high prevalence of HIV and HCV infection has been found in our results.

A strong association between genotype 1b and the development of a severe liver disease were detected.

The genotypes vary distribution depends on the co-infection with HIV, and that knowledge would be useful to the prognosis and treatment of the HCV infection.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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