Genotypic structure and susceptibility to antimicrobial agents of Acinetobacter baumannii and Pseudomonas aeruginosa in Russian burn intensive care units
Abstract number: 1734_158
Goncharov A., Solomenny A., Aslanov B.
Objectives: At first we studied the epidemic situation and the genetic structure of hospital population of Acinetobacter baumannii developed (during 20022004 yrs) in two burns intensive care units in St. Petersburg and Leningrad region. We observed in these hospitals chronic epidemy of an acinetobacter infection linked to contamination by bacteria from mattresses and functional beds on an ``air pillow''. The aim of this study was to investigate the genotypic structure and susceptibility to antimicrobial agents and bacteriophages of MDR A. baumannii and P. aeruginosa.
Methods: A total of 56 clinical isolates of A. baumannii and 11 isolates of P. aeruginosa from 2 burn ICUs (BICU 1 and BICU 2) were characterised with respect to antimicrobial agents and bacteriophages susceptibility. 17 bacteriophages of A. baumannii and 12 bacteriophages of P. aeruginosa have been isolated from clinical specimens and tested for their antimicrobial activity.
Results: Typing by RAPD-PCR with the M13 primer showed the hospital population of Acinetobacter presented as a restricted number of clonal lines, four (13, 10, 5 and 4 isolates) in BICU 1 and two (7 and 6 isolates) in BICU 2, circulating for the prolonged time (>2 yrs.). No identical amplification pattern was indicated in the clone predominating in BICU 1 and BICU 2. Class 1 integron variable segments (2.5 kb in size) were determined in predominating in BICU 1 clonal line. Prevalence of resistance to cefaclor, cefotaxime, ceftriaxone, ceftazidime and gentamicin was significant in BICUs strains. The integron-positive isolates were sensitive to imipenem or polymyxin only. Only one clonal line of P. aeruginosa was detected in two burn ICUs using RAPD-PCR. 44.4% of acinetobacteria and 75.0% of P. aeruginosa were susceptible to bacteriophages.
Conclusion: The large-scale researches on search and selection of bacteriophages, for their application as a therapeutic agent are necessary.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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