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Evaluation of Helicobacter pylori stool antigen test for the detection of H. pylori infection in paediatric patients

Abstract number: 1734_110

Gonzalez Mediero G., Ramos J.M., García J.I., Otero Varela I., Del Blanco T., Sanchez Conde J.A.

Objective:Helicobacter pylori plays an important role in the pathogenesis of some several extra or intragastroduodenal diseases. The assessment of H. pylori antigen in human stools has been proposed as a valuable non-invasive diagnostic tool. The aim of this study was to evaluate the usefulness of H. pylori stool antigen (HpSA Premier Platinum HpSA, Meridiam Diagnostics, Cincinnati, USA) in diagnosis of H. pylori infection in children patient.

Methods: 1270 patients have been consulted in the Gastroenterology Paediatric unit as news patients during a period of 4 years (1/01/02–31/12/05). Seventy two patients aged 3–15 years with chronic pain abdominal and suspicion of infection for H. pylori were studied in this work; sixty one (4.8% of total patients) were included in this study and eleven discarded for not advice. Stool samples were taken before antibiotic treatment. The patients were evaluated in two stages, the first one for symptoms as pattern of pain abdominal chronic (per-umbilical, epigastria) and study for HpSA and the second one with gastrointestinal gastroscopy for assessment study mucosa for histology, ureasa test and serology (ELISA). Patients were defined as H. pylori positive infection if histology was confirmed as gastritis chronic antral and H. pylori was showed in the biopsy.

Results: 31 patients were positive for H. pylori (50.8%). The media optical densities at 450 nm were 1.088 (range 0.064–3.500) and 0.088 (0.004–0.444) in the patients positive and negative for H. pylori antigen respectively. The sensitivity and specificity of method were as follows respectively; ureasa 86.3% and 89.9%, serology 63% and 78% and HpSA was 99% and specificity 40.3%. Predictive value of positive and predictive value of negative test respectively is ureasa 96% and 75%, serology 85% and 67% and HpSA 51% and 98%.

Conclusion:Helicobacter pylori antigen test is non-invasive, easy to perform and it can be a test to exclude negative cases when the result is negative, but it can not be considered as a diagnostic test in paediatric group in our area in Vigo (Galicia, Spain). We considered that positive patients who gastrointestinal gastroscopy was negative, must be revised and to evaluate the evolution and prognosis. Until October 2006 none of them were request consultant or revision.

Acknowledgements: All test for Helicobacter pylori has been performed by Elena Saavedra as technical personal.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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