Liaison® VZV IgG and VZV IgM assays: a comparative study
Abstract number: 1734_105
Huynen P., Melin P., Hayette M., Mathieu J., De Mol P.
Varicella-zoster virus (VZV) belongs to the Herpesvirus family. Varicella is the full-blown primary infection and zoster is caused by the reactivation of latent VZV. Varicella and zoster are mainly diagnosed clinically but serology plays an important role, especially when the course of disease is non-typical.
Objectives: VZV serology is currently carried out by microplate analysers in our institute. The aim of this study was to evaluate if the LIAISON® VZV IgG and VZV IgM (DiaSorin, Saluggia, Italy), two fully automated immunoassays, based on chemiluminescence technology (CLIA) could be an alternative method, quick and easy to perform, whose performance meets our current quality requirements. We therefore performed a comparative evaluation and investigated the overall agreement between LIAISON® VZV IgG and IBL VZV IgG ELISA (Immuno Biological Laboratories, Hamburg, Germany) as well as between LIAISON® VZV IgM and Enzygnost VZV IgM ELISA (Dade Berhing Enzygnost, Marburg, Germany).
Methods: The performances of LIAISON® VZV IgG and IBL VZV IgG ELISA were compared for a total of 165 selected routine serum samples (139 positive, 19 negative, 7 indeterminate). For interpretation of results of the LIAISON® assay, a cut-off value of 150 mIU/mL was used, in order to achieve the highest diagnostic specificity and sensitivity. Discordant results were solved by Euroimmun VZV IgG (Euroimmun AG, Luebeck, Germany) ELISA.
The performances of LIAISON® VZV IgM and Enzygnost VZV IgM ELISA were compared for a total of 160 selected routine serum samples (22 positive, 114 negative, 24 indeterminate). Discordant results were solved by Euroimmun VZV IgM and NovaTec VZV IgM ELISA (NovaTec Immunodiagnostica GmbH, Dietzenbach, Germany).
Results: The comparative study between LIAISON® VZV IgG and IBL VZV IgG ELISA as well as between LIAISON® VZV IgM and Enzygnost VZV IgM ELISA showed overall agreement of 89.7% and 80% respectively. When Euroimmun VZV IgG, Euroimmun VZV IgM and NovaTec VZV IgM tests were used and that a consensus between at least two out of three tests was applied, those values were 96.4% and 97.5% respectively.
Conclusions: The LIAISON® VZV IgG assay is a valid alternative for the quantitative detection of VZV IgG antibodies, since the kit performance is at least equivalent to that of the kits currently available on the market.
With its high specificity, the LIAISON® VZV IgM test, a fully automated method, is also a good alternative for the detection of IgM antibodies.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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