Genomic diversity of Leishmania parasites isolated by RAPD-PCR
Abstract number: 1734_94
Nomanpour B., Ardehalli S., Motazedian M., Sobhani N.
Objectives: Cutaneous leishmaniasis (C.L.) is prevalent in different parts of world. Clinical manifestations of cutaneous leishmaniasis vary from a simple to multiple Lesions and chronic forms. Studies have revealed the presence of genetic diversity among leishmania parasites. For better understanding of these diversities characterisation and phylogenic study seem to be necessary.
Methods: In the present study. 82 isolates of cutaneous lesions from different provinces of Iran were cultured on suitable media and characterised by using 8 primers. DNA was extracted for further studies using RAPD-PCR. DNA fingerprints of various isolates were studied by calculating inter and intra-species polymorphisms bands and also genetic distance by Jacquard's distance coefficient.
Results:Leishmania major and L. tropica were isolated and characterised from Esfahan, Tehran, Pars and Kerman provinces; L. major from Khuzestan province; L. infantum from Boushehr and Estern Azerbaijan provinces.
Leishmania tropica characterised from Yazd, Khorasan and Golestan provinces where no characterisation of the organisms has previously been reported.
Phylogenic studies demonstrated five main groups within L. tropica strains.
A strain isolated from a chronic patient with nine years history of CL in Tehran had 0.35 genetic distances as compared to others and showed some of L. infantum polymorphism bands. The possibility of this isolate being a hybrid can not be ruled out.
Leishmania major isolates comprised four main groups by maximum genetic distance of 0.40. A strain of L. major isolated from an Afghan refugee had genetic distance of 0.40 which differed from those isolated and studied from Iran.
Three strain of L. infantum isolated from Boushehr and Azerbaijan (Meshkin shahr) provinces had genetic distance of 0.37 compared with the standard strain. The inter-train of genetic distance among these organisms were 0.27. The reason for such a difference may be due to different source of standard strain. (Borkinafaso)
Conclusion: Hybridisation seems to be a form of genetic exchange among Leishmania species and strains. Geographical proximity, population mobility and the nativity of species and strains may be among the factors involved in possible genetic exchange.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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