Antimicrobial susceptibility and AP-PCR typing of Acinetobacter spp. strains isolated from three teaching hospitals, Tehran, Iran

Abstract number: 1734_92

Sadeghifard N., Zaeimi Yazdi J., Ranjbar R., Ahmadi A., Ghasemi A., Rostamzad A.

Objectives: This study was designed to establish antimicrobial susceptibility and AP-PCR typing of Iranian isolates of Acinetobacter spp.

Methods: During this study, 67 Acinetobacter isolates (including 21 A. baumannii and 46 non-Acinetobacter baumannii strains, were obtained from three University hospitals in Tehran. The site of these isolates were included the blood, urine, wound, and respiratory tract. Their susceptibility to 17 antibiotics was tested and then all A. baumannii isolates were typed by AP-PCR method.

Results: Results showed that all Acinetobacter baumannii isolates were resistant to multiple antibiotics including cefoprazone, ceftazidime, ticarcillin/clavulanic acid, aztreonam, meropenem, ceftizoxime, carbenicillin, cefixime, ceftriaxone, ticarcillin, cephotaxime, but were sensitive to colistin. The results of molecular typing by AP-PCR method demonstrated 7 different patterns in the isolates.

Conclusion: Our study as the first report in Iran, established AP-PCR assay as a molecular typing method for multi-drug resistant clinical Acinetobacter baumannii strains. Upon our results, because of the presence and spread of multi-drug resistant Acinetobacter spp. in the hospitals, more care should be taken for preventing them from producing nosocomial infections in Iran.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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