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Efficacy of a commercial multiplex herpes viruses PCR in the viral diagnosis of different clinical samples

Abstract number: 1734_89

Reina J., Ruiz de Gopegui E., Mena A., Macia M.

Objectives: To know the efficacy of a commercial nested PCR in the aetiological diagnosis of infections caused by the herpesviruses family in different clinical samples.

Material and Methods: The different studied samples have been analysed by means of a commercial herpesviruses (HSV, VZV, CMV, EBV and HHV-6) multiplex nested PCR (Real, Durvitz, Valencia, Spain) following the instructions of the manufacturer.

Results: During the period 2000–2006, 1,445 PCRs have been made; being considered as positive 170 (11.7%). The percentage of positivity have varied widely in the study period from 8% (2003) to 17% (2001), as the number of clinical samples studied, 115 in the 2000 to 316 in 2005 (p < 0.05). The viruses amplified were: citomegalovirus (CMV) 90 (52.9%) (p < 0.05), herpes simplex (HSV) 55 (32.5%), varicella-zoster (VZV) 19 (11.1%), Epstein-Barr (EBV) 4 (2.3%) and human herpesvirus type 6 (HHV-6) 2 (1.1%). The clinical sample most studied was the CSF with 1.087 (75.2%) (p < 0.05) from patients with the clinical diagnosis of aseptic meningoencephalitis; being positive 57 (5.2%), corresponding to 36 HSV (63.1%) (p < 0.05), 14 CMV (24.5%), 5 VZV (8.7%) and 2 HHV-6 (3.5%). The colon biopsies of patients with colitis or Crohn's disease have represented 117 samples (8.1%), being positive 53 (45.2%), corresponding 45 CMV (84.9%), 4 HSV and 4 EBV. 93 bloods have been studied (6.4%), being positive 13 (13.9%), corresponding to 12 CMV (92.3%) and 1 VZV. The ocular punctions (aqueous specimens) represented 44 samples (3.1%), being positive 14 (31%), corresponding to 7 CMV (50%), 4 HSV (28.5%) and 3 VZV. Other analysed samples were 29 ammniotic fluids (2%) with 4 positives (13.7%), 2 CMV and 2 VZV; 23 Bronchoalveolar lavage, BALs (1.5%) with 13 positives (56.5%), 7 CMV (53.8%), 3 HSV and 3 VVZ. In the other 52 (3.5%) diverse samples, 16 positive were detected (30.7%), corresponding to 8 HSV (50%), 5 VVZ (31%) and 3 CMV (19%).

Conclusions: The commercial nested PCR studied has shown a high diagnostic efficacy specially in the samples of central nervous system (CSF) and in the diagnosis of viral encephalitis. This method must be recommended in all those samples in which a low viral load exists.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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