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Clonality among high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical samples

Abstract number: 1734_41

Soltan Dalal M., Hasibi M., Aghaei S., Mohajer Iravani B., Pourshafie M.

Objectives: During recent years, nosocomial infections caused by enterococci have increased in different countries. Unfortunately enterococci which acquire high level gentamicin resistance (HLGR) become non curable by using the common effective antibiotictherapy protocoles.

Methods: We determined the prevalence of HLGR among clinical enterococcal isolates with disc diffusion method by using Gm (120mg) disk. 726 enterococcal strains were collected from different hospitals and clinical laboratories. Clonality relation between HLGR strains were determined by PFGE and Ribotyping techniques and AAC (6')-APH(2") genotype detection was performed by PCR method.

Results: The species distribution percents was 8.5% for E. faecium and 91.5% for E. faecalis. These figures in HLGR strain were 61.6% and 38.4 for E. faecalis and E. faecium respectively. PFGE, ribotyping and PCR techniques classified these isolates in different genotypes.

Conclusion: Our results suggest dissemination of genetically related HLGR strains in nosocomial infection and indicate that high prevalence of HLGR enterococcal colonisation is an important problem in our medical centres.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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