Emergence of PER-1 extended-spectrum b-lactamase-producing Acinetobacter baumannii clinical isolate in Bulgaria

Abstract number: 1734_37

Strateva T., Ouzounova-Raykova V., Todorova A., Mitov I.

Objectives: Strains of Acinetobacter baumannii producing the extended-spectrum b-lactamase (ESBL) PER-1 are widespread in Turkey and Korea, and have also been reported from France and Belgium. Here we describe the emergence of strain of A. baumannii producing PER-1 ESBL in Bulgaria.

Methods: A strain of A. baumannii 255–00 was isolated on 11 February 2000 from the wound of a male patient aged 65 years in a Clinic of surgery in Sofia. Antimicrobial susceptibilities were detected by the disk diffusion method and Etest (AB Biodisk, Solna, Sweden) and interpreted according to the NCCLS-2004 recommendations. The ESBL-production was evaluated by the double-disk synergy test. Polymerase chain reaction (PCR) amplification and sequencing of blaPER-1 were performed.

Results: The studied strain of A. baumannii was resistant to ampicillin/sulbactam, carbenicillin, piperacillin, ceftazidime, cefoperazone, cefepime, amikacin and gentamicin, but remained susceptible to imipenem, meropenem, ciprofloxacin and polymyxin B. A synergic ``ghost zone'' was observed between the ceftazidime 30 mg disk and the amoxicillin/clavulanic acid 20/10 mg disk. Using PCR, this A. baumannii isolate was identified as PER-1-positive. PER-1 enzyme-mediated resistance was confirmed by sequencing.

Conclusions: The present work illustrates the inter-country spread of PER-1-producing A. baumannii isolates. To our knowledge, this is the first report of PER-1 in clinical isolate of A. baumannii in Bulgaria.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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