Evaluation of antibacterial activity and biofilm formation in Klebsiellapneumoniae in contact with essential oil and alcoholic extract of cumin seed (Cuminum cyminum)
Abstract number: 1733_1577
Derakhshan S., Sattari M., Bigdeli M.
Introduction:Klebsiellapneumoniae has special importance in nosocomial infection. Because of the acquisition of multiresistant plasmids, these organisms are resistant to a number of antibiotics, including extended-spectrum cephalosporins and aminoglycosides.
Methods: In this study essential oil of cumin seeds (Cuminum cyminum) obtained in a cleavenger system by hydrodistillation method. Antibacterial activity of essential oil and alcoholic extract of cumin against Klebsiellapneumoniae ATCC13883 and clinical Klebsiellapneumoniae (ceftazidime-resistant strain) were evaluated on the minimum inhibitory concentration by the broth-dilution method. Synergistic or antagonistic effect with antibiotic disks were tested in agar media involving sub-MIC concentration of oil and alcoholic extract. Biofilm assay were performed by colorimetric method as described by O'Toole in 1998.
Results: The MIC was 1/800 fold of essential oil for K. pneumoniae ATCC 13883 and 1/200 for clinical K. pneumoniae. Also MIC was 1/32 fold of alcoholic extract for two strain in sub-MIC concentration, synergistic effect was seen between ciprofloxacin and oil (significant increasing of inhibition zone diameter) and antagonistic effect was seen between trimethoprim and sulfamethoxazol (reversion of colonies around the antibiotic disk) for the clinical K. pneumoniae. The decrease or prevention of biofilm formation occurred when oil concentration increased. Alcoholic extract was low effect in antibiotical activity and biofilm formation. The oil was analysed by GC and GC/MS. Main constituent in oil of Cuminum cyminum is cuminaldehyde (4 isopropylbenzaldehyde).
Discussion: The results of this study suggested that the essential oil and alcoholic extract of cumin seed could be used in medicinal industries (desinfectant or antiseptic), but in-vivo tests are required to ascertain fully their medicinal properties and potential toxicity.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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