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Use of multilocus enzyme electrophoresis to examine genetic relationships among isolates of Klebsiellapneumoniae

Abstract number: 1733_1356

Rahmati M., Etemadi G., Asadi S., Abdi-Ali A., Feizabadi M.

Objectives: Multilocus enzyme electrophoresis was carried out in order to epidemiological studies and to evaluate the genetic relationships of Klebsiellapneumoniae in Tehran. The characterisation of electrophoretic variants of metabolic enzymes reflects allelic variations at the corresponding structural gene locus, and defines as electrophoretic types (ET) which correspond to genotypes. The aim of this study was to determine the antibiotic susceptibility pattern, detection of ESBLs and analyse the genetic relationships between the strains by Multilocus Enzyme Electrophoresis (MEE). No information to date is available on the population genetic analysis of K. pneumoniae in Iran

Methods: 100 isolates of K. pneumoniae were collected from different clinical samples in two hospitals in Tehran. In addition to antibiotic susceptibility testing, Extended spectrum b-lactamase enzymes were detected by both Double Disk Synergy Test (DDST) and Phenotype Confirmatory Test (PCT). Population genetic of isolates was determined by Multilocus Enzyme Electrophoresis.

Results: Of 100 isolates, 56% and 20% were from urinary and respiratory tracts infections, respectively. The other 24% were from other sources such as blood, wound, CSF, ear, eye, vagina, bone and stool infections. The highest resistance of isolates was to ampicillin (97%) and aztreonam (77%), respectively. All strains were susceptible to imipenem and meropenem. 46% of isolates were ESBLs positive and the resistance of these strains to ampicillin and cephalexin was 97.83% and 80.43% respectively. Analysis of the strains with 17 metabolic enzymes produced 51 electrophoretic types (ETs) There was more than one strain in 13 ETs, whereas other strains classified in separate ETs. Genetic diversity among strains varied from leucin-tyrisin peptidase (0.733) to Glucose 6-phosphate dehydrogenase (0.387). Mean genetic diversity among these strains were determined 0.515.

Conclusion: This present work shows high rate of infections with ESBL strains in Tehran. MEE revealed the close relationship among isolates of K. pneumoniae from patients at Tehran hospitals. However polymorphic loci such as leucin-tyrosin peptidas with a high genetic diversity (0.733) were observed, MEE differentiated the strains with ESBL phenotype. MEE as a chromosomal marker is a reliable technique for typing and discriminating of bacterial population.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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