The analysis Y. pestis strains from Kazakhstan and USA by PFGE method

Abstract number: 1733_1346

Meka-Mechenko T., Tashmetov R., Atshabar B., Nekrassova L.

Objectives: Y. pestis strains from plague foci of Republic of Kazakhstan differ on phenotypic properties. The differences in plasmid profile and structure of genes are marked also. The comparative analysis Y. pestis strains isolated from the natural plague foci of Kazakhstan and USA by PFGE method are of interest.

Methods: Are investigated by PFGE method Y. pestis 48 strains isolated in 1938–2002 from the natural plague foci of Kazakhstan and 12 strains from USA. Strains are isolated from fleas, ticks, wild rodents, domestic animals and from the plague patients.

Results: Y. pestis strains isolated from autonomous plague of Central Asian desert natural focus, Volga-Ural steppe autonomous focus, Volgo-Ural sandy natural focus have identity at the genetic level from 82.6% up to 100%, biotype of these strains is mediaevalis. Only 1 strain from Ustyurt autonomous focus (strain's biotype is antigua) is generically close (84.5%) to strain from Tian-Shanian natural plague focus (Sarydzhas autonomous focus). Two plague strains by genetic structure are closer to strains isolated in USA (83%) in comparison with others strains isolated from plague foci of Kazakhstan (77.2%).

In strains isolated from Sarydzhas autonomous focus (Tian-Shanian natural focus) percent of similarity rather low – 77.2%. One strain with positive fermentation of glycerine and negative denitrification 95.7% of similarity of genetic picture with strain isolated from Moinkum autonomous focus (Central Asian desert natural plague focus). Others investigated strains are typical and percent of genetic identity makes 84.5%. The similarity of genetic structure strains isolated from USA varies from 88.8% up to 100%. Rather low percent of identity plague strains isolated in plague foci of Kazakhstan (77.2% up to 100%), is caused landscape-epizootological feature of sites and characteristic properties of circulating populations of the activator of plague.

Conclusion: The identity plague strains on genetic level depend on geographical region and from strain's biotype. In strains concerning to one biotype and isolated from one natural plague focus percent of identity at molecular level is high. The received results of computer analysis and dendrogram of phylogenetic connections between investigated strains allow to determine their origin that increases efficiency of epidemiological of supervision of plague.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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