Distribution of Ure C, cagA and vacA genes in Helicobacter pylori isolated from patients with gastroduodenal disease in Tehran, Iran
Abstract number: 1733_1339
Jafari F., Aslani M., Shokrzadeh L., Baghai K., Dabiri H., Zojaji H., Razavilar V., Kharaziha P., Haghazali M., Molaei M., Zali M.R.
Objective: The aim of this study was to investigate the prevalence of H. pylori infection and the frequency of UreC, cagA and vacA genes in H. pylori infection in Iran.
Methods: A total of 280 gastric biopsies from patients who were qualified for endoscopies of the upper gastrointestinal tract from February 2006 to September 2006 were included in this study. These samples were cultured for H. pylori by conventional methods. The genomic DNA was extracted from biopsy samples by standard method. UreC (glmM) gene was amplified by polymerase chains reaction (PCR), then genotyping was done based on cagA and vacA (s1/s2/m1a/m1b/m2) genes.
Results: Out of 280 biopsy samples, 179 (63.9%) patients with gastroduodenal disease were infected with H. pylori. The number of positive culture in patients with gastric cancer and duodenal ulcer were 8 (4.5%) and 27 (15.1%) respectively. The cagA gene was amplified in 133 (74.3%) of the isolated strains. The frequency of vacA genotypes for s1 (signal region) and m2 (middle region) genotypes were 63.5% and 61.2%, respectively. In 67 (38%) of strains s1m2 genotype was found.
Conclusion: The study demonstrated that vacA s1and m2 genotypes dominant in Iranian strains were also predominant strains in North America (6070%), but not in Middle-East (39%), suggesting the relation between Iranian strains and European strains. The VacA s1 genotype is the most frequent genotype and there was a correlation between infections with H. pylori strains carried cagA gene. Moreover vacA s1/m2 genotypes found the frequent genotype responsible for the development of gastroduodenal diseases.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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