On-site molecular typing of Pseudomonas aeruginosa as an adjunct to infection control in a paediatric cystic fibrosis centre
Abstract number: 1733_1337
Lucas C., McMillan M., Brown D., Williams C.
Introduction: Cystic Fibrosis (CF) is a genetically inherited disease affecting around 1 in 3,000 children and young adults in the UK. It is characterised by a progressive decline in pulmonary function, exacerbated by the acquisition of specific micro-organisms, in particular Pseudomonas aeruginosa. To date, no cure is available and the life span of sufferers is directly influenced by the effectiveness of local infection control practices. However, at the present time, detection of cross-infection episodes is entirely dependent on the genotyping service provided off-site.
Methods: Isolates of Ps. aeruginosa grown from CF patients were collected throughout 2005. An in-house molecular typing method using the Random Amplification of Polymorphic DNA (RAPD) technique was developed and genetic profiles of the isolates were analysed compared using BioNumerics v.4.0 computer software.
Results: DNA extracts from 90 Ps. aeruginosa strains grown from 19 CF patients have been analysed using RAPD. Strains determined to be unique to a single patients by the Reference Laboratory clustered together, demonstrating >80% homology using RAPD. Transmissible strains known to be genotypically related, which were cultured from different patients, demonstrated a similar degree of homology. The advantage for infection control purposes is the rapid availability of results with on-site molecular screening.
Conclusions: Initial data indicate that RAPD allows recognition of unique Ps. aeruginosa strains and allows discrimination between diverse strains, to a level which is adequate for routine screening purposes. Introduction of on-site RAPD typing will clarify the relatedness between local strains, hasten detection of cross infection episodes and facilitate timely and effective infection control action.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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