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Moxifloxacin versus ampicillin+gentamicin in the therapy of experimental Listeria monocytogenes meningitis

Abstract number: 1733_1285

Yamazhan T., Sipahi O., Turhan T., Pullukcu H., Calik S., Tasbakan M., Sipahi H., Ulusoy S., Arda B.

Objective:Listeria monocytogenes (LM) is an important pathogen causing meningitis in immunocompromised and and immunocompetent host, The main therapeutic choice is ampicillin+gentamicin which is shown to be synergic in experimental meningitis model in rabbits, Moxifloxacin is effective against LM in vitro and in machrophages, There is no human or animal study comparing ampicillin+gentamicin versus moxifloxacin in LM meningitis, In this study it was aimed to compare the antibacterial activity of moxifloxacin and ampicillin+gentamicin in the treatment of LM meningitis in rabbit meningitis model.

Methods: Meningitis was induced by direct inoculation of a clinical strain isolated from an immunocompromised patient (107cfu/mL), into cisterna magna of New Zealand rabbits, After 16 h of incubation time, rabbits were separated into three groups moxifloxacin (M), ampicillin+gentamicin (A) and control, (C), Group M received 20 mg/kg moxifloxacin (Bayer, Germany) at the end of the incubation time and 5 h later by IV route, Ampicllin (Mustafa Nevzat, Turkey, 30 mg/kg/h) and gentamicin (IE Ulagay, Turkey, 2.5 mg/kg/h) were also given by IV route with continuous infusion for 8 h in 36 cc 0.9% NaCl and group C did not receive any treatment, CSF samples (0.1–0.25 mL) were obtained 16 h and 24 h after induction of meningitis, At the end of the study period (24 h after the induction of meningitis), animals were humanely killed by intravenous infusion of high dose nembutal, The bacterial count in CSF was measured by standard serial dilutions of 10 mL CSF in 0.9% NaCl and incorporation into sheep blood agar (Oxoid, Basingstoke, UK) pour plates, Data were evaluated by SPSS 11.0 package programme using Mann-Whitney U-test, Kruskal-Wallis test and Fisher's c2 test, A P-value less than 0.05 was considered significant.

Results: At the end of 16 h of incubation time, CSF bacterial counts were similar in all groups (p > 0.05), When three groups were compared (table 1), bacterial counts in both treatment groups 24 h after the induction of meningitis were significantly lower (p < 0.05) than control group, When two treatment groups were compared bacterial counts were similar (Table 1).

Table 1. Bacterial counts in CSF of rabbits during study period.

GroupNo. of rabbitsLog10 cfu.mL
16 h after induction of meningitisEnd of treatment   
Group C95.309±0.4616.346±0.623
Group M95.375±0.3563.830±0.518
Group A75.512±0.7124.193±0.794

Conclusion: These data suggest that antibacterial activity of moxifloxacin is similar with ampicillin+gentamicin in the treatment of experimental LM meningitis of rabbits

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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