Antimicrobial biodegradable composition based on high-molecular weight polyvinylpyrrolidone for the prophylaxis of experimental osteomyelitis

Abstract number: 1733_1276

Afinogenov G., Tikhilov R., Mironenko A., Bogdanova T., Afinogenova A., Kozlov I., Krasnova M., Anisimova L.

Objectives: The problem of the post-surgical osteomyelitis prophylaxis after metal-osteosuture is still actual now. The local application of collagen with gentamycin used to prolong the antibiotic activity in tissues of wound area. Modern technologies allow to obtain high-molecular weight PVP with different biodegradation terms to use it as a matrix for various medicines.

The aim was to evaluate the efficacy of antimicrobial composition of amikacin with activator and polyvinylpyrrolidine as a biodegradable prolonging agent for the post-surgery infections prophylaxis.

Methods: The rabbit shin-bone was bared under the aseptical intravenous anaesthesia and two bone defects were formed with 0.1 N NaOH to obtain surface necrosteosis. The microbial culture of S. aureus (0.5 ml of 2×0⁸ CFU/mL) was introduced into the bone defects twice before and after the K-wires were placed. 5 ml of amikacin with activator and PVP were placed directly on the bone and wound area before made a suture. The total condition, body temperature and wound view were valued. The animals were sacrificed on the 7th, 14th and 21st days after the surgery. The clinical evidences of irritation, the pus and tissue necrosis presence were valued as well as bacteriological and histological researches were made with muscle and marrow biopsies and implanted K-wires.

Results: An acute osteomyelitis with expressed tissue microbial contamination was obtained in the control group. The animals with prophylaxis introduction of antibacterial composition didn't display clinical evidences of osteomyelitis. The tissues microbial contamination proved to be the same during the experiment in the control group. The experimental animals showed a reliable decreased microbial contamination of tissues involved in infectious process. The whole eradication of S. aureus was obtained by the 21st day in muscle and marrow biopsies. Testing the K-wires the microbial growth was displayed in control group and only by the 7th day – in experimental group. The bactericidal composition showed a residual antimicrobial activity in animal tissues during the experiment.

Conclusions: The data obtained show the efficacy of the amikacin combination with activator based on high-molecular weight polyvinylpyrrolidine and an opportunity to it's application for the post-surgery infections prophylaxis in traumatology, orthopaedics and other surgery areas.