Investigation on efficacy of garlic extract (Allium sativa) against burn wound infection by Pseudomonas aeruginosa in burned animal model
Abstract number: 1733_1273
Erzanlou M., Arab R., Alaei R.
Objective: Despite advances in medical cares, infections are the major causes of death in patients with sever burn. Gram negative rod, P. aeruginosa, is among the leading causes of burn wound infection. The situation for patients with P. aeruginosa infections is particularly problematic, because this organism rapidly evolves to multi-drug resistant forms and spreads through hospital wards and burn units. So it could not be eradicated readily with common antibiotic therapies.
Methods: Burn was induced on the back of anaesthetised animals by heated brass bar, after the hair removal. Bacterial infection was established by topical applying of highly pathogenic clinical isolate of P. aeruginosa. Potential of garlic extract on reduction of mortality was evaluated by topical application of 10 percent (V/V) garlic extract on burned and infected animals, and was compared with two control groups: burned and infected animals either treated with topical silver sulfadiazine (SSD) (1%) or left untreated. The same groups were subjected to evaluate bacterial counts in organs (blood, liver, spleen and skin).
Results: Our results indicated that topical administration of garlic extract (10%) extended the survival of mice for 36 days, compared with survival of the untreated group. Both garlic extract and SSD treatments reduced the microbial loads in vital organs (blood, liver, spleen), compared to that of untreated control group (P < 0.05).
Conclusion: The preliminary results presented in this study suggest that garlic extract may offer a promising and novel means for the treatment of P. aeruginosa burn wound infection and open new insight into further investigation on this area.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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