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Looking for evidence of Chlamydia pneumoniae presence in atherosclerotic and non-atherosclerotic segments of arteries obtained from autopsies at a forensic unit in a Kuala Lumpur hospital, by both rtPCR to detect omp A VD4 base sequences and immunohistoch

Abstract number: 1733_1235

Puvaneswaren P.S., Mustafa M.I.A., Abdullah N.B., Inuzukam M., Haque Q., Mansor A.H.

Background and Objective: A plethora of conflicting reports regarding the link between Chlamydia pneumoniae (CP) and atherosclerosis exist, however few are available from Malaysia and Southeast Asia. This study was performed to look for evidence of CP presence in atherosclerotic as well as non-atherosclerotic arteries of the same human cadaver.

Methods: Both a molecular method using real-time PCR to look for the presence of VD4 sequences, a highly conserved domain of CP ompA gene, as well as immunohistochemical method using CP species-specific antibody ab13941 purchased from DakoCytomation were conducted. Amplification of human beta actin gene, a housekeeping gene, acted as an endogenous control. Fifty-two atherosclerotic arterial segments and thirty-three non-atherosclerotic segments were obtained from 33 fresh cadavers at the Forensic Unit, Hospital Kuala Lumpur for the purpose of the study.

Results: Both the immunohistochemistry assay as well as the real-time PCR assay failed to detect any C. pneumoniae from all the tested tissue samples.

Conclusion: Thus, this study could not demonstrate any molecular or immunohistochemical evidence for the presence of CP in any of the arterial tissues tested whether atherosclerotic or non-atherosclerotic.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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