The activity of five commonly used topical antiseptic agents on epidemic and sporadic strains of Acinetobacter baumannii

Abstract number: 1733_1205

Demertzi E., Avraamides G., Azadian B., Hill R.L.R., Turton J., Pitt T.L.

Objectives:Acinetobacter baumannii is increasingly being recognized as a cause of outbreaks of opportunist infections in intensive care patients in hospitals. These organisms contaminate the inaminate environment and may survive for prolonged periods on surfaces. Contact with these surfaces by healthcare personnel may lead to contamination of their hands and facilitate the transmission of the organisms to others. A number of topical antibacterial agents are used for hand hygiene or as surgical disinfectants and so we questioned whether selected agents were equally effective against strains of A. baumannii prevalent in British and European hospitals.

Materials and Methods: A panel of 30 strains was assembled comprising 11 characterised epidemic strains (SE clone, Oxa-23 clones 1 and 2, European clones, etc.) 12 representatives of more minor outbreak strains, and 7 sporadic isolates. The following products (active agent) were tested:

1. Purell gel (62% ethyl alcohol),

2. Desderman gel (78.2% ethanol + 0.1% phenylphenol),

3. Sterzac bath liquid (2% Triclosan, isopropyl alcohol etc.),

4. Hydrex (0.5% chlorhexidine gluconate w/v in 70% v/v DEB),

5. Betadine (10% povidone-iodine).

Agents were tested for activity using the prEN 12054 European standard method. Standardised bacterial test suspensions (1 mL) were mixed with products (9 mL) and survival at 30 s, 1 min and 5 min was determined by viable counts on agar.

Results: The minimum requirement for bactericidal activity for compliance with prEN 12054 is a 10(5)-fold reduction in viable count within 1 min. Each product tested fulfilled prEN 12054.

We conclude that A. baumannii are killed by commonly used topical antiseptics and hand hygiene should be effective in limiting their spread in hospitals.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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