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In vitro activity of three echinocandins (caspofungin, micafungin and anidulafungin) against Aspergillus spp.

Abstract number: 1733_1177

Martos A.I., Martin de la Escalera C., Lopez-Oviedo E., Aller A.I., Romero A., Serrano C., Valverde A., Martín-Mazuelos E.

Objective: Echinocandins are a novel class of antifungal agents with an excellent activity against Aspergillus spp. The in vitro activity of caspofungin (CF), micafungin (MF) and anidulafungin (AF) were tested against clinical isolates of Aspergillus spp.

Material and Methods: Forty-one clinical isolates of Aspergillus spp. were used in the study: 28 isolates of A. fumigatus, 10 isolates of A. flavus and 3 A. terreus. The susceptibility testing to these three echinocandins were performed by the broth microdilution (MD) procedure according to the CLSI M-38A document. The ranges of drugs dilution were 0.03–8 mg/L. Inoculum suspensions were prepared recovering the conidia from a 7-day culture growth on Potato Dextrose Agar at 35°C (final inoculum: 0.5–5×104 CFU/mL). MECs (Minimal Effective Concentrations) were determined and the readings were performed at 48 h (Kurtz et al. 1994, AAC 38: 1480–1489). Candida parapsilosis 22019, C. krusei 6258, A. fumigatus 204305 and A. flavus 204304 were included as Quality Control strains.

Results: MECs50, MECs90 and ranges of MECs (mg/L) of CF, MF and AF are shown in Table 1. The MECs were ≤ 0.25 mg/mL for all strains tested. MF and AF shown similar MECs (≤ 0.03 mg/L) while CF MECs range were from 0.03 to 0.25 mg/L. The Quality Control strains were within the published range.

Table 1. 

SpeciesaMEC (mg/L)
CaspofunginMicafunginAnidulafungin
RangeMEC50MEC90RangeMEC50MEC90RangeMEC50MEC90 
A. fumigatus (28)<=0.03–0.250.060.125<=0.03<=0.03<=0.03<=0.03<=0.03<=0.03
A. flavus (10)<=0.03–0.250.060.06<=0.03<=0.03<=0.03<=0.03<=0.03<=0.03
A. terreus (3)0.125  <=0.03  <=0.03  
Total (41)<=0.03–0.250.060.25<=0.03<=0.03<=0.03<=0.03<=0.03<=0.03
aNumber of isolates given in parentheses.

Conclusion: These three echinocandins shown an excellent in vitro activity against all clinical isolates of Aspergillus spp. tested.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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