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Role of exposure to HPV infection and genetic susceptibility for cervical neoplasia in Italy

Abstract number: 1733_1058

Agodi A., Barchitta M., La Rosa N., Cipresso R., Guarnaccia M., Cultrera M., Caruso M., Castiglione M.G., Ettore G., Travali S.

Objectives: Although cervical infection sustained by high-risk human papillomaviruses (HPV) is considered a necessary event in the development of cervical cancer, it is clear that it is not sufficient. Thus, other factors are likely involved in influencing the persistence and progression of an HPV infection towards malignant disease. One such influence may be genetic susceptibility, although this issue continues to be a focus of debate. A cross-sectional study was conducted in order to: i) estimate the prevalence of cervical HPV infection and ii) investigate the role of the p53 codon 72, GSTM1 and GSTT1 polymorphisms in cervical neoplasia.

Methods: During the period 2004–2006, all women attending the Service for Colposcopy of an Italian Hospital were consecutively enrolled. Cases were women with high-grade cervical intraepithelial lesion (H-SIL). Controls were women without a history of cervical abnormalities or with low-grade cervical intraepithelial lesion (L-SIL). The presence of various HPV types was examined using the HPV Screen Kit (Nuclear Laser Medicine). Polymorphisms of p53 gene were evaluated with PCR with primers encompassing the polymorphic site at codon 72. The GSTM1 and GSTT1 genetic polymorphisms were evaluated using multiplex PCR techniques. In all subjects, gene polymorphisms were determined and results were analysed according to HPV infection status and histological feature, in comparison to the control group.

Results: A total of 232 women were enrolled. The overall HPV prevalence was 71.1% patients. High-risk types were found in 89.7% of infected women and multiple infections in 47.9% of infected women. HPV 16 was by far the most common type (51.5% of HPV positive women), followed by HPV 6 (32.1% of HPV positive women), and HPV 56 (29.7% of HPV positive women); type 18 was found in only 2.4% of HPV positive women. No significant differences in the p53 and GST polymorphisms distribution were found in relation to the infection with HPV. On the contrary, our study showed a significant association between the arginine homozygous genotype and H-SIL, corresponding to an odds ratio of 5.6 (95% CI: 1.515–20.880).

Conclusion: Our study showed a higher HPV prevalence compared with other European populations. Besides, it revealed a possible susceptibility role of the p53 codon 72 polymorphism at an early carcinogenetic stage in cervical cancer. However further research is needed with close attention to study design and methodological features.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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