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Human metapneumovirus in paediatric viral respiratory infections

Abstract number: 1733_1053

Alexandre J., Alves M., Rocha G., Magalhães-Sant'Ana A., Meliço-Silvestre A., Nogueira C.

Objectives: Respiratory infection in infants and young children is a significant public health problem worldwide. Infection with respiratory syncytial virus (RSV) is the most common known cause of lower respiratory tract infection in young children. Human metapneumovirus (hMPV), a member of Pneumovirinae subfamily of the Paramyxoviridae family, first discovered in 2001, has recently been described as a casual agent of acute respiratory disease. The virus is widely distributed and has been detected in Japan, China, Australia, USA and several European countries, and is responsible for about 2 to 25% of cases of acute respiratory tract infections in infants. This relatively high incidence and the fact that hMPV associated disease may be severe, emphasized the need for a reliable, sensitive and rapid diagnostic test for detection of hMPV.

Methods: From November 2005 to October 2006, 157 respiratory samples were collected from paediatrics patients with symptoms of respiratory tract disease. For the hMPV study total RNA was extracted by MagNA Pure Compact (Roche) using the MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche), according to the manufacter's instructions. cDNA was synthesized with random hexamer primer from the Transcriptor cDNA synthesis Kit (Roche) according to the manufacter's instructions. The detection of hMPV was performed by a real time PCR assay using primers targeting the nucleoprotein gene (N) and a fluorogenic endonuclease oligoprobe (Taqman) for detection. Samples were also tested for screening and identification of Adenovirus (ADV), Parainfluenza (P) 1, 2 and 3, and RSV by an indirect immunofluorescence assay (Respiratory Virus Panel, Biotrin) according to the manufacter's instructions.

Results: hMPV was detected in 20 (13%), RSV in 87 (55%), ADV in 6 (4%), P3 in 3 (2%), respiratory samples. Co infections were also identified: 3 hMPV +/RSV + and 1 RSV +/ADV+.

Conclusions: RSV is still the leading cause of respiratory tract illness in infants and children and our findings agree with detections rates (50–90%) published in literature. Nevertheless, hMPV was found in a significant number of cases (13%) as reported by other authors.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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