The distribution of human papillomavirus infection in the male urogenital tract in Russian urological patients
Abstract number: 1733_1025
Smelov V., Gorelov A., Pleijster J., Savicheva A., Morre S.
Objectives: The prevalence of symptomless genital human papillomavirus (HPV) infection among men is not well established. This is partly due to a poor understanding of which sites should be tested. Up to now there is no published study regarding the HPV distribution in Russian male population. HR-HPV types are accepted to be involved in the aetiology of cervical cancer and this has lead to new vaccine approaches. The influence of HPV infection on the risk of cancers among men, i.e. prostate cancer, is still under investigations. The goal of this study was to investigate the HPV infection presence in the urethral and prostate samples in the Russian urological patients.
Methods: The urethral and prostate samples of 210 men (mean age 31.7, range 1660 years), who visited 2 urology clinics in St-Petersburg, were sequentially collected from May through to October 2006 to be tested against sexually transmitted infections and HPV. No specific selection was made to the obtained this study group.
The presence of high- and low-risk HPV (HR- and LR-HPV, respectively) DNA in the distal urethra and expressed prostate secretion (EPS) was investigated in Amsterdam by the GP5+/6+ PCR assay followed by subsequent HR- vs LR-HPV typing using EIA (enzyme immunoassay). The oligoprobe cocktails for 24 HR-HPVs (i.e., 16, 18, 31, 33, 35) and 21 LR-HPVs (i.e., 6, 11, 32, 40, 42) were used for the group-specific detection.
Results: The results are presented in the Table. The HPV distribution in the urethra and prostate was detected as 16.2% and 13.3% for HR- and 6.2% and 5.7% for LR-HPV infection, respectively. Concordance between LR- and/or HR-HPV distribution in both anatomical sites was observed in only 2025%. We observed ``grey zone'' values in 5.7%; these samples will be re-tested.
Conclusions: The presence of HR- and LR-HPV types in the distal urethra and EPS of men was investigated and compared in this study for the first time.
The total HR- and LR-HPV prevalence was detected in this group as 23.3% and 10.0%, respectively; with a low concordance rate between the two anatomical sites studied.
At the moment we are in progress to: (1) determine HPV types distribution; (2) extend the study group; and to investigate the HPV distribution in men with: (3) co-infection, i.e. Chlamydia trachomatis, and (4) chronic urological disease, i.e. chronic prostatitis.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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