Evaluation of PlateliaTM Lyme IgM and PlateliaTM Lyme IgG assays for Lyme borreliosis diagnosis
Abstract number: 1733_1000
Jaulhac B., De Martino S., Tabouret M.
Objectives: Lyme borreliosis (LB) is a non contagious infection caused by Borrelia burgdorferi sensu lato and is transmitted by ticks of the Ixodes genus. It is the most prevalent tick-borne zoonosis and is considered as an important emerging infection in Europe, North America and Far Eastern countries. Due to limitations of direct detection, antibodies screening remains the mainly used method to support a clinical diagnosis of LB. Objective of the study is to evaluate the performances of Platelia Lyme IgM and Platelia Lyme IgG (Bio-Rad Laboratories) for an accurate diagnosis of Lyme disease.
Methods: Platelia Lyme IgM and IgG assays are two step assays using respectively immunocapture and indirect ELISA formats. Obtained results were compared to Enzygnost® Borreliosis IgM and IgG (Dade Behring).
Specificity was calculated on 200 healthy blood donors from eastern region of France and on 206 patient samples with potential cross-reactive conditions (syphilis, leptospirosis, rheumatoid factor, CMV or EBV infections, etc...). Sensitivity was determined on 70 confirmed LB sera from early and late stages of the disease.
Results: By comparison with reference ELISA methods, specificities on blood donors were 99.0% and 99.5% for respectively IgM and IgG assays. On potential interfering samples, specificities were 94.6% for IgM and 97.5% for IgG (100% and 97.8% for syphilis IgM and IgG positive samples). Sensitivity results are presented in the table below for assays considered individually or in combination.
Conclusion: Performances of Platelia Lyme assays are adequate for diagnosis of infected LB patients and fulfill recommended criteria for screening. Using same protocols, both IgM and IgG assays can be run in parallel.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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