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Prevalence of hepatitis B virus genotypes in patients with chronic hepatitis B virus infection in Madrid

Abstract number: 1733_984

Fernandez-Olmos A., Mercadillo M., Moreira V., Mateos M.

There is evidence that HBV genotypes play a role in several aspects of HBV infection, such as disease profile or response to treatment. The eight genotypes of HBV (A-H) have a distinct geographic distribution.

Objectives: To study the prevalence of hepatitis B virus genotypes among hepatitis B chronic patients and their relationship with age and the presence of HBeAg and anti-HBe in serum.

Methods: HBV genotypes were determined in serum from 76 patients, by direct sequencing of a 137-nucleotide fragment from the S region of the HBV genomal (Trugene HBV Genotyping kit, Bayer Health Care, New York, USA).

Results: Prevalence of HBV genotypes in the 76 cases was as follows: 41 genotype D (53.9%), 23 genotype A (30.3%), 8 genotype F (10.5%) and 4 genotype E (5.3%). After stratification of patients by age, we found that genotype D was significantly more prevalent among patients over 45 years (71.4% vs. 37.5%, P = 0.021) Comparison of the distribution of the most common genotypes, A and D, showed that genotype D was present more often in HBeAg(-) (ratio A:D = 0.5) than in HBeAg(+) (A:D = 1.6), although the difference was not significant (P = 0.142). Distribution of genotypes F and E was similar among the two groups.

Conclusion: Genotype D is the most common HBV genotype found in our patients in Madrid, in keeping with other studies in Western Europe. Interestingly, genotype F was found in a high number of Spanish patients and this finding points to the introduction of strains from South America to the Spanish population and posibly to Europe due to the high immigration rate. In contrast, no genotype E was found among autochthonous patients. All four genotype E infected patients were immigrants from Africa. Our study did not include Asian patients, therefore, genotype B or C were not found as expected. We did not find genotype G. In agreement with other studies, we conclude that genotype D is the most prevalent, the presence of genotypes E and F may be due to demographic movements and genotype G is uncommon in Spain. As stated by other authors genotype D was more prevalent than A in patients aged over 45 years.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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