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Isolation of a canine-like human rotavirus strain G3P[3] from a child with acute gastroenteritis hospitalised in Palermo, Italy

Abstract number: 1733_939

De Grazia S., Giammanco G.M., Ramirez S., Cascio A., Martella V., Arista S.

Objectives: Group A rotavirus are the most common aetiologic agents of acute gastroenteritis in children and infants worldwide. They are ubiquitous viruses, also infecting the young of many animal species. There are a number of reports of atypical human rotavirus strains, that show some genetic and antigenic features of rotavirus isolated from various animal species. Such features suggest that the occurrence of rotavirus genetic reassortment and interspecies transmission may occur in nature. In the present study, we report the molecular analysis of VP7, VP4, VP6 and NSP4 genes of an unusual human rotavirus strains G3P[3], detected in a young child with acute gastroenteritis in Palermo, South of Italy, during 1997.

Methods: The rotavirus strain PA260 was isolated from a 2 year-old child admitted with severe diarrhoea at the ``G. Di Cristina'' Children's Hospital of Palermo, Italy during an epidemiological surveillance study. As it showed a G3 type, VP7 related, a P, VP4 related, not determinable type, and a subgroup I specificity, it was considered atypical and it was submitted to VP7, VP4, VP6 and NSP4 characterisation by sequence analysis.

Results: The viral strain was characterised as G3P[3] rotavirus, showing a 99% and 98% identity in the VP7 and VP4 proteins, respectively, to the canine rotavirus RV52/96 strain isolated in Bari in 1996. Also the analysis of VP6 and NSP4 proteins revealed an high amino acid identity, 95% and 98.8% respectively, of PA260/97 to the canine RV52/96 strain. No human G3 strain, available in GenBank, appeared as closely related to PA260 strain as RV52/96 strain.

Conclusions: Since the VP7, VP4, VP6 and NSP4 sequences of the PA260/97 strain are located very close to canine RV52/96 strain sequences, it is likely that the infection of the Italian child was the result of canine-to-human transmission. The PA260 strain is the third rotavirus strain with G3P[3] type described throughout the world and the first in Italy.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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