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Evaluation of fluorescence in situ hybridisation for the identification of clinically relevant Candida species

Abstract number: 1733_914

Poppert S., Lakner A., Bartel M., Essig A.

Objectives: Rapid identification of Candida albicans is highly warranted in order to adapt antifungal therapy. Aim of the study was to establish and evaluate a Fluorescence in situ Hybridisation (FIAH) assay for this purpose.

Methods: We evaluated FISH using a previously published and a newly developed C. albicans probe for the identification of C. albicans in comparison to API 20 C, the chromogenic Candida ID-Agar and determination of filamentous colony morphology. 17 reference strains, 182clinical isolates and 41 blood cultures showing yeasts in the Gram stain were included.

Results: All tests produced highly concordant results. The Candida ID-Agar however was not able to discriminate C. albicans and C. dubliniensis. Reliable identification of C. albicans by API and by determination of filamentous colony morphology took 48 hours. FISH allowed identification of C. albicans within 3 hours with a specificity and sensitivity of 100% from subculture as well as directly from blood cultures.

Conclusion: As a robust, simple and over all extraordinarily fast method, FISH is an appropriate tool for the identification of C. albicans isolates, especially from blood cultures.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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