Environmental contamination in the rooms of MRSA-colonised patients
Abstract number: 1733_819
von Baum H., Raychevska F., Möricke A.
Objectives: The presence of MRSA in the rooms of MRSA colonised patients and its relationship to the contamination of healthcare workers' (HCW) gloves and clothing was examined.
Methods: 10 standardised environmental samples from patients' rooms as well as samples from the gloved hands and clothing of HCW were examined. Samples were incubated for 48 h at 37°. Presumptive positive colonies were confirmed as S. aureus. Resistance to methicillin was determined using the Slidex® MRSA Detection (bioMérieux). All environmental MRSA isolates as well as the corresponding patients' isolates were genotyped using pulsed-field-gel-electrophoresis (PFGE).
Results: MRSA was present in the rooms of 18 of 25 MRSA patients (72%). Contamination of HCWs' gloved hands and/or clothing was identified in 15 of 25 nurses (60%); 3 of 6 physicians (50%), 3 of 3 physiotherapists (100%) and 3 of 15 members of the housekeeping staff (20%). In the majority of patients more than one MRSA strain was present.
Environmental MRSA strains indistinguishable from the strains colonising the patients were detected in only half of the cases.
Conclusion: Healthcare workers must be aware that contamination of their gloved hands and clothing whilst caring for a MRSA colonised patient is highly probable. In the environment of MRSA colonised patients not only the patients' own strains but additional genotypically distinct MRSA strains were detected.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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