Trichosporon asahii in hospitalised patients

Abstract number: 1733_785

Kanellopoulou M., Velegraki A., Adamou D., Martsoukou M., Milioni A., Skarmoutsou N., Papafrangas E.

Trichosporon asahii is implicated in a variety of clinical manifestations ranging from simple superficial cutaneus to severe invasive disease.

Objectives: To assess the incidence of T. asahii in clinical specimens from patients with different underlying conditions and to retrospectively compare the molecular subtypes of isolates in a period of three years (2002–2005) in a tertiary Greek hospital.

Patients and Methods: Isolates originated from 12 males and 5 females, (mean age 69 years) with cerebral completed stroke (n = 5), diabetes mellitus (n = 4), ureterostomy-nephrostomy (n = 3), renal dysfunction (n = 2), COPD (n = 2), rheumatoid arthritis (n = 1). Indwelling catheter was present in all patients. T. asahii identification was performed by API ID32C, bioMérieux, France). Complementary biochemical and physiological tests confirmed characterisation of the strains. PCR fingerprinting by a minisatellite specific primer M13 [5'-GAGGGTGGCGGTTC-3'] was used for strain delineation.

Results: During the study period, 17 T. asahii strains were isolated from an equal number of patients. Fifteen strains were recovered from urine specimens, and two from respiratory secretions. The two patients with T. asahii isolated from respiratory secretions were intubated. Eleven strains were isolated from the same clinic at different time intervals. Minisatellite fingerprinting grouped the in time and space related isolates in a single cluster.

Conclusion: (1) Although T. asahii is classed as an emerging opportunistic agent, in this study was exclusively associated to colonisation of the urinary tract. (2) Risk factors for colonisation included indwelling/endotracheal catheter, diabetes mellitus, and old age. (3) The low T. asahii prevalence among these high-risk groups of patients over a period of three years is in accordance with the prevalence recorded internationally. (4) Pending examination of a larger sample size, M13 minisatellite fingerprinting can be recommended for rapid prospective identification of hospital outbreaks.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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