Transmission of Pneumocystis jirovecii from the grandparent immunocompetent carriers to his susceptible granddaughter

Abstract number: 1733_784

Rivero L., Montes-Cano M., de la Horra C., Respaldiza N., Friaza V., Munoz-Lobato F., Morilla R., Medrano F., Varela J., Calderon E.

Objectives:Pneumocystis jirovecii causes pneumonia in immunocompromised host but was first identified as an interstitial pneumonia involving premature and malnourished children. Asymptomatic forms of Pneumocystis infection have been described in immunocompetent persons like patients with chronic respiratory diseases who could play a role for the transmission to susceptible persons. Clusters of Pneumocystis pneumonia (PCP) cases among immunosuppressed patients suggesting person to person transmission have been described. This study was undertaken to determine if PCP could have been caused by familiar transmission.

Methods: A six months old female was hospitalised for acute bilateral pneumonia associated with chronic dry cough. Diagnostic test revealed severe malnutrition and PCP. Oral wash samples were collected from: mother, father, brother, grandmother and grandfather who referred a history of rheumatoid arthritis and chronic bronchitis respectively. None of them had symptoms at the moment of the study. DNA was extracted using a commercial kit. The mt LSU rRNA gene was identified by nested-PCR; polymorphism at nucleotide position 85 and 248 were stablishing by direct sequencing.

Results: The mt LSU rRNA gene of P. jirovecii was identified in three cases: the infant, the grandmother and the grandfather. The child and grandparents showed the same cluster of P. jirovecii, genotype 1 (85C/248C) was identified in the three cases, and genotype 3 (85T/248C) was also detected in the grandfather.

Fig. 1. Probable transmission of P. jirovecii infection from grandparents to infant.

Conclusion: Our findings support a probable transmission of Pneumocystis infection from the grandparent asymptomatic carrier to his susceptible granddaughter.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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