Prevalence of thermotolerant Campylobacter species in broilers, eggs, chicken abattoir and human samples in a Hungarian county

Abstract number: 1733_748

Kardos G., Mészáros J., Galántai Z., Turcsányi I., Bistyák A., Juhász Á., Damjanova I., Pászti J., Kiss I.

Objectives: We monitored the prevalence of thermotolerant campylobacters in chickens and chicken-derived food by following a broiler premise to the abattoir as well as a laying stock and the eggs produced. We also examined the incidence of human campylobacteriosis in Hajdú-Bihar county at the same period.

Methods: Study period was April to October 2006. Twelve pools of five droppings were taken at the age of one, three and immediately prior to slaughter from five consecutive stocks of a broiler premise. A laying stock was sampled bimonthly. From the latter fifty and six pools of five commercial and reject eggs, respectively, were examined weekly. Environmental and abattoir samples were collected following the official guidelines. Human data originated from routine faecal samples, only individuals older than one year were considered. Animal, egg, environmental and abattoir samples were enriched in Preston broth and inoculated onto CAT or CCDA agar. Human samples were inoculated onto solid medium directly. Identification of isolates was performed using genus- and species-specific PCRs.

Results: Environmental as well as chicken feed samples were always negative. Pooled faecal samples collected from three- and six-week-old broilers were almost uniformly culture as well as PCR positive, excepting the April rotation. Those collected at the age of one week were negative. All isolates, excepting two Campylobacter coli, were C. jejuni, though PCR revealed four cases of mixed carriage. All sample sets from layer hens were also positive, but not all individual pools; C. jejuni, C. coli and mixed carriage were found in approximately equal proportion. Out of 300 reject eggs 17 C. jejuni and 8 C. coli, while out of 570 pools of five commercial eggs 10 C. jejuni and 2 C. coli were isolated. From 70 meat samples (including 30 collected from retail shops) 35 C. jejuni and 23 C. coli isolates were obtained. Twenty C. coli isolates originated from processing of one stock. Two faecal samples taken from abattoir staff yielded 2 C. jejuni isolates. Human outbreaks were not detected, the incidence was 2–5%, the monthly proportion of C. coli was between 13% and 36%.

Conclusions: Both eggs and broilers were contaminated by campylobacters, leading to high contamination of meat, which also passed to the shops. Human incidence could be lowered considerably by decreasing Campylobacter carriage of broilers and laying hens.

The work was supported by the GVOP-3.1.1.-2004–05–0472/03 grant.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Location: ICC, Munich, Germany
Presentation type:
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