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Isolation and characterisation of Salmonella from Turkish avian food samples

Abstract number: 1733_738

Avsaroglu D., Jaber M., Akcelik M., Bozoglu F., Schroeter A., Guerra B., Helmuth R.

Objective: To assess the prevalence of Salmonella (S.) in avian food samples from Turkey and to characterise the strains according to their serotypes, phage types and antimicrobial resistance (R) patterns.

Methods: 76 chicken meat samples were collected from different markets in Ankara, Turkey (2005–2006). Strains were isolated according to ISO 6579. Biochemical tests were performed with the API20E system. Serological analyses, and phage typing of S. Enteritidis and S. Typhimurium, were carried out. All isolates were tested for their susceptibility to 16 antimicrobial agents by the disk diffusion method. All resistant isolates were further tested by broth microdilution and for an extra panel of 11 b-lactams, as well.

Results: 45% of the chicken meat samples analysed (34 out of 76) were positive for Salmonella. From these samples, 71 isolates were obtained. After phenotypic characterisation, they were considered as 42 epidemiologically unrelated strains. The 42 strains belonged to 12 different serotypes. The most prevalent was S. Enteritidis (10 strains, 24%) followed by S. Infantis and S. Virchow (7, 17% each); S. Group C1 with antigenic formula [6,7: k: -] (5); S. Kentucky, S. ssp. I rough form (3); S. Thompson (2); S. Agona, S. Corvallis, S. Nchanga, S. Senftenberg, and S. Typhimurium (1). Among S. Enteritidis, phage type PT21 was predominant (6 of 10), followed by PT6, PT1 and PT3 (2, 1, and 1 strain). Forty per cent (17 strains) of the strains were resistant to some antimicrobial agent. From these, 13 strains were multiresistant and four monoresistant. The most frequent resistance was to nalidixic acid (36% of all strains, 88% of the resistant). All these strains showed also decreased susceptibility to ciprofloxacin. Multidrug resistance to more than 6 antimicrobials was observed in S. Infantis (6 strains showed KAN-NEO-NAL-STR-SPE-SUL-TET-TMP-SXT resistance), and S. Virchow (1 strain AMP-CEF-NAL-STR-SUL-TMP-SXT). This Virchow strain showed resistance to several extended-spectrum b-lactams. All S. Enteritidis were susceptible, except one PT21 NAL-resistant strain.

Conclusion: Almost half of the food samples analysed were contaminated with Salmonella, especially serotypes S. Enteritidis, S. Infantis and S. Virchow. Antibiotic resistance is also a problem in the contaminated food from avian origin (40% resistance). The quinolone resistance in all serotypes and multi-resistance in S. Infantis and S. Virchow need further attention.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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