Antimicrobial susceptibility and phage types of Salmonella typhimurium human strains isolated during 20022005 in the Slovak Republic
Abstract number: 1733_620
Majtánová L., Majtán V.
Objectives:Salmonella Typhimurium is a common cause of human salmonellosis in many countries. This is the second most common salmonella serovar isolated from human sources. The extensive use of antimicrobials in human and veterinary medicine has led to an increase in multidrug resistance strains (MDR). A MDR S. Typhimurium strain, with definitive phage type 104 (DT104), has emerged and spread over the world since 1984. The aim of this study was to analyse the antimicrobial resistance patterns of 330 S. Typhimurium human isolates and to investigate the distribution of their phage types.
Methods: Antimicrobial susceptibility tests were performed by disk-diffusion method using 10 different antibiotic disks according to the method of CLSI/NCCLS. Phage types were determined by the extended phage typing scheme of Anderson et al. (1977).
Results: Of total 330 strains analysed, MDR was identified in 192 (58.2%) isolates. They were the most frequently resistant to ampicillin, streptomycin, sulfisoxazole, tetracycline, chloramphenicol, sulfamethoxazole-trimethoprim and trimethoprim. None of the all isolates were resistant to ceftriaxone and only one isolate was resistant to ciprofloxacine. Three hundred and thirty strains encompassed 25 definitive phage types, 38 strains were atypical types (RDNC) and 76 ones were untypable. The predominant phage types were DT104 (22.4%), DT20a (22.1%) and DT120 (5.6%).
Conclusion: The strains studied expressed MDR and belonged to different phage types. The MDR was ascertained mainly in the predominant phage types DT104, DT20a, DT120 and also in untypable strains originating from sporadic cases of infection. Continual surrveillance is necessary to monitor the dissemination of these and similar MDR strains as well as their phage types in order to implement appropriate control measures.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
|Back to top|