New emerging human infection caused by Bartonella vinsonii ssp. arupensis in Russia
Abstract number: 1733_548
Kruglov A., Barmina G., Osadchaya V., Frolova G., Markov A., Smirnov G., Bashkirov V., Kirillov M., Kosoy M.
Objective: First case of human illness caused Bartonella vinsonii ssp. arupensis was described in Wyoming, 1999. The cattle rancher suffered from bacteraemia and high fever. The second case was recorded in France in 2005 where a patient was hospitalised with endocarditis.
Methods: Surveyed 95 patients with infective endocarditis (IE) and a fever of unknown origin (FUO) in the clinics of Moscow Medical Academy during 20042006 years. The basic lab methods of research were: inoculation of blood samples to Vero E6 cell culture and incubation at 37°C for 10 days; culturing of samples on plates with hard medium in an atmosphere of 5% CO2; estimation of antibody titers by indirect immunofluorescence (IFA; PCR with genus-specific primers; RFLP and sequence of obtained amplicones.
Results: High frequency of the positive serological reactions to bartonellae antigenes was detected (10.3% IE, 5.1% FUO). Two cases of cultural positive B. vinsonii ssp. arupensis infections were detected. Both patients were young women. First had FUO. Second IE. Blood samples from the patients were obtained on day 39 (1st case) and on day 73 (2nd case) after admission. Main clinical manifestations of the illness were: the acute onset of disease and the symptoms of expressed intoxication. The patients did not have typical clinical attributes of classical Bartonella infection; the inflammation traits in blood were slightly expressed, and low-grade bacteraemia symptoms were observed; the disease had positive dynamics of after the nonspecific antibacterial therapy. Typical colonies appeared on agar plates after 7 days of incubation at 37°C in 5% CO2 atmosphere. Small Gram-negative bacteria were seen after staining. Biochemical properties and antimicrobial susceptibility of the both strains were studied and found typical for Bartonella genus. Antibodies titers to B. vinsonii in serum of both patients were 1/64.
In both cases PCR from bacterial isolates with Bartonella-specific primers resulted in strong positive signals with 5 targets such as partial sequences of the gltA, ribC, ftsZ, 23S rRNA, and sucB genes. RFLP and sequence analysis demonstrated that both strains belong to B. vinsonii ssp. arupensis.
Conclusion: The high frequency of positive serological reactions to Bartonella amongst patients with IE was detected. This is a first report about the bartonelloses caused by B. vinsonii ssp. arupensis in humans in Russia.
|Session name:||European Society of Clinical Microbiology and Infectious Diseases|
|Location:||ICC, Munich, Germany|
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