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Streptococcus pneumoniae strains isolated from laboratory animals and pets

Abstract number: 1733_490

van der Linden M., Al-Lahham A., Seegmüller I., Nicklas W., Kopp P., Reinert R.

Objectives:Streptococcus pneumoniae is the major causative pathogen of many childhood community-acquired respiratory tract infections (RTIs), including community-acquired pneumonia, acute otitis media and acute maxillary sinusitis. In the present study S. pneumoniae strains isolated from mastomys, guinea pigs, mice, rats and a cat were characterised.

Methods: Species diagnosis was performed using optochin- and bile solubility testing. Serotyping was performed using the Neufeld Quellung reaction. MICs to antibiotics were determined with the microdilution method according to the CLSI recommendations. Multilocus Sequence Typing was performed according to standard methods.

Results: Between 1986 and 2006 S. pneumoniae was isolated from 33 laboratory animals during routine control checks in the animal facility in the German Cancer Research Center and 2 pets treated in a medical veterinary laboratory. Isolates were obtained from mastomys (n = 24), rats (n = 4), guinea pigs (n = 3), mice (n = 3) and a cat. S. pneumoniae were isolated from nose, lung, trachea, abdomen and eye. Only four animals showed disease symptoms. Two guinea pigs were C4-immune deficient and suffered from severe peritonitis. The third guinea pig and the cat were pets and had severe respiratory problems. Serotypes detected were: 14 (n = 29), 19F (n = 3), 33A (n = 2) and 7C (n = 1). One isolate was rough and two were non typable. The guinea pigs isolates had serotypes 19F. All isolates were sensitive to penicillin, telithromycin and levofloxacin. One isolate was clarithromycin resistant. Twenty-six isolates were ST 15 (serotype 14 and n.t.) a sequence type commonly found in human isolates. The cat isolate was ST 180 (serotype 3). Twelve isolates had new MLS-types, seven of which are close variants of known MLSTs. The three guinea pig isolates showed the same completely new combination of known alleles.

Conclusions:S. pneumoniae strains could be routinely isolated from non-human hosts, showing animals to be a reservoir for S. pneumoniae.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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