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Evolution of antimicrobial susceptibility of Campylobacter jejuni strains isolated from hospitalised children in Athens, Greece

Abstract number: 1733_423

Papavasileiou H., Papavasileiou K., Chatzipanagiotou S., Makri A., Andrianopoulou I., Voyatzi A.

Objective: To investigate the current epidemiology and the antimicrobial susceptibility of Campylobacter jejuni strains, isolated from children with gastroenteritis.

Material and Methods: 170 strains were isolated from stools of children aged up to 14 years during a two year period (2004–2005). The specimens were plated on charcoal, blood free, selective medium (oxoid, UK) and incubated for 48 h at 42°C in a microaerophilic atmosphere (BBL). The identification was performed by typical growth, microscopic examination, hippurate hydrolysis, positive catalase and oxidase reactions. For the susceptibility of Campylobacter the diffusion disk method was used and the determination of MIC was performed by E-test, (AB, Biodisk, Solna Sweden) for the following antibiotics: erythromycin, amoxicillin + clavulanic acid, ampicillin, gentamicin, tetracycline, ciprofloxacin, clindamycin. As control, the ATCC33560 strain was used. Results were interpreted according to the criteria of the NCCLS for Enterobacteriaceae. The serotyping was performed, based on Penner's heat-stable (HS) serogroups, in the Greek reference laboratory.

Results: From the 170 strains of C. jejuni isolated from gastroenteritic cases, 30% showed resistance to ciprofloxacin (MIC ≥ 4 mg/l), 55% to tetracycline (MIC > 8 mg/l), 13% to clindamycin (MIC ≥ 8 mg/l), 4% to ampicillin (MIC > 16 mg/l), 6% to erythromycin (MIC ≥ 8 mg/l) and 4% to amoxicillin + clavulanic acid (MIC ≥ 16/8 mg/l). In gentamicin all strains were sensitive.

The MIC50 and MIC90 of the strains are shown in the table.

StrainMIC (mg/L)
MIC50MIC90 
Erythromycin0.502
Ciprofloxacin0.50>32
Gentamicin0.500.50
Clindamycin0.2532
Tetracycline16,256  
Ampicillin24
Co-amoxiclav0.502

Most of the strains resistant to ciprofloxacin were also to tetracycline. Only two strains found to be multiresistant. Two serotypes were more predominant: HS: 4, 13, 16, 43, 50 and HS: 1,44, while a significant number of strains was non-typeable (NT).

Conclusion: Resistance of C. jejuni to erythromycin, ampicillin, amoxicillin + clavulanic acid, still remains low, whereas to tetracycline and ciprofloxacin increases. As long as resistance of C. jejuni to quinolones increases, verification of antimicrobial susceptibility is suggested as a routine practice in laboratories.

Session Details

Date: 31/03/2007
Time: 00:00-00:00
Session name: European Society of Clinical Microbiology and Infectious Diseases
Subject:
Location: ICC, Munich, Germany
Presentation type:
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